| 초록 |
We present the monovalent cations effect on the binding properties of G-quadruplex when TMPyP bound to multiple planar GGG tetrads. Monovalent cations like potassium and sodium have been shown to act as a stabilizing factor for the G-quadruplex structural formation. Once G-quadruplex formed by monocations, some small molecule ligands can also effect on the stability of G-quadruplex-binding molecule complexes. On the basis of the melting assay for G-quadruplex-TMPyP in K+/Na+ buffered solutions at a low ratio (R=0.2) is closely resemble to G-q alone. Which suggests that the presence of small amount of TMPyP has a minimal effect on the thermal stability of quadruplex. As a results of the job plots for G-quadruplex with TMPyP in K+ buffer, major inflection point for quadruplex-TMPyP complexes, at x = 0.70 was observed. Based on the various spectroscopic results, different monovalent cations can take an effect on the binding properties of the G-quadruplex-Porphyrin complexes. |
