| 초록 |
We present the base sequence effect on the binding properties of G-quadruplex and porphyrin complexes. Change of base composition in loop or at termini of sequences have a significant effect on the stability of final structure. These resulting formations can also effect on the binding properties between G-quadruplex and porphyrin. According to results, once an extra T-base (-TTT-, S2) was placed on loop in sequence 1(-TT-, S1), Their CD spectra was quite different to each other. In contrast, when A-base (5'-A…-TTT-, S3) was added at termini of 5'-side of the sequence 2(-TTT-, S2), there is not induced any structural changes against to S2. On the melting profiles, whether extra base is placed on loop or at termini of 5'-side, less stable than the sequence that without extra base. On the basis of the various spectroscopic results, base composition can effect on the binding properties of porphyrin-quadruplex complexes including their stabilities. |
