| 초록 |
Poly(ethylene glycol) has been prominently used as the basis of solid supports via microfluidics due to their non-fouling properties and compatibility with various solvents. However, limitation regarding its low loading efficiency still remains. Hence, we sought to quantitatively evaluate the efficiency of various types of amino-functionalized PEG-based microbeads by analyzing and comparing each: 1) purity of a synthetically designed Jung-Redemann (J-R) decapeptide, 2) cell morphology of NIH-3T3 and 3) cell viability on Arg-Gly-Asp (RGD tripeptide)-bound resins. Furthermore, we suggested the extra synthesis of Fmoc-Lys(Fmoc)-OH on each type of resins to assess its applicability in solid phase peptide synthesis (SPPS). We anticipate such method to be useful not only in bio-assays but also in determining the characteristics of unknown synthetically designed peptides. |
