| 학회 |
한국공업화학회 |
| 학술대회 |
2016년 봄 (05/02 ~ 05/04, 여수 엑스포 컨벤션) |
| 권호 |
20권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Stable Isotopic Labeling-Based Quantitative Targeted Glycomics (i-QTaG) |
| 초록 |
Mass spectrometry analysis combined with stable isotopic labeling is a prospective method for the relative quantification of aberrant glycosylation in diseases and disorders. We developed a stable isotopic labeling-based quantitative targeted glycomics (i-QTaG) technique for the comparative and quantitative analysis of total N-glycans using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The i-QTaG method was applied to direct comparison of the total N-glycan profiles between normal human sera (n=8) and prostate cancer patient sera (n=17). The intensities of the N-glycan peaks from the i-QTaG method showed a linearity (R2＞0.99) with the amount of the glycoproteins. Then the ratios of relative intensity between the isotopically 2-AA labeled N-glycans were close to the theoretical molar ratios. We also demonstrated that the up-regulation of the Lewis antigen (∼82%) in sera from prostate cancer patients. |
| 저자 |
김경진, 황철환, 박한규, 김윤곤
|
| 소속 |
숭실대 |
| 키워드 |
MALDI-MS; N-glycan; comparative quantitation; stable isotopic labeling
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| E-Mail |
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