| 학회 |
한국공업화학회 |
| 학술대회 |
2009년 봄 (04/15 ~ 04/17, 호텔인터불고(대구)) |
| 권호 |
13권 1호 |
| 발표분야 |
정밀화학 |
| 제목 |
Development of Chemical Tools for Studying a Novel Glycan-dependent Signaling Pathway |
| 초록 |
Dynamic O-GlcNAcylation is an important signaling mechanism akin to phosphorylation. Deregulation of this pathway has been linked to insulin resistance and several neurodegeneration. We developed a labeling approach that has now provided a general means for O-GlcNAc proteome. Attachment and detachment of this O-GlcNAc are carried out by two key enzymes which are O-GlcNAc transferase and O-GlcNAcase, respectively.We developed a high-throughput assay with a highly sensitive fluorogenic hexosaminidase substrate for O-GlcNAcase. This substrate was further optimized to give selectivity for O-GlcNAcase over other related hexoaminidases.We also generated novel inhibitors of O-GlcNAcase with excellent selectivity and potency.Such inhibitors can induce non-insulin-dependent diabetes in model systems making them invaluable research tools. |
| 저자 |
김은주1, John A. Hanover2
|
| 소속 |
1대구대, 2Kidney Diseases |
| 키워드 |
O-GlcNAcylation; Insulin resistance; O-GlcNAcase; O-GlcNAc transferase
|
| E-Mail |
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