| 학회 |
한국화학공학회 |
| 학술대회 |
2004년 봄 (04/23 ~ 04/24, 공주대학교) |
| 권호 |
10권 1호, p.473 |
| 발표분야 |
생물화공 |
| 제목 |
Production of Antithrombotic Hirudin in GAL1-Disrupted Saccharomyces cerevisiae |
| 초록 |
A gratuitous strain was developed by disrupting the GAL1 gene (galactokinase) of recombinant Saccharomyces cerevisiae harboring the antithrombotic hirudin gene in the chromosome under the control of the GAL10 promoter. A series of glucose-limited fed-batch cultures were carried out to examine the effects of glucose supply on the hirudin expression in the gratuitous strain. Controlled feeding of glucose successfully supported both cell growth and hirudin expression in the gratuitous strain. The optimum fed-batch culture done by feeding glucose at a rate of 0.3 g h-1 produced maximum hirudin concentration of 62.1 mg l-1, which corresponded to a 4.5-fold increase compared with a simple batch culture done with the same strain. |
| 저자 |
박용철1, 김명동2, 이태희1, 임형권3, 서진호1
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| 소속 |
1서울대, 2Columbia Univ., 3목암생명과학(연) |
| 키워드 |
Hirudin; Saccharomyces cerevisiae; Galactose; GAL1; Gratuitous
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| E-Mail |
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| 원문파일 |
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