| 초록 |
A biodegradable polyurethane, which is classified as a psuedo poly(amino acid), has been developed using L-tyrosine (LTU2a). Using water-in-oil-in-water emulsion, nanoparticles encapsulating complexes of DNA and linear polyethylenimine (LPEI) or bovine serum albumin labeled with fluorescein have been fabricated. In addition, their surfaces have been decorated with PEG. The morphology of these nanoparticles, which was confirmed with microscopy and TEM, is spherical. The DNA release exhibited a first order kinetics. Furthermore, a successful transfection of LTU2a nanoparticles by hepatic cancer cell (HepG2) has been confirmed with confocal laser microscope. These characteristics are ideal for drug-delivery systems designed to transport and release drugs in the cytoplasm. Therefore, LTU2a nanoparticles could be a promising for gene-delivery applications that require sustained release of DNA for the duration of a cell’s lifetime. |
