| 초록 |
Recently, many researches looking at attention biorefinery have focused on identification of novel and efficient cellulases from various microorganisms and high expression of them because of their valuable potential in industrial applications, most notably biofuel and biochemical production. Among the microorganism, fungi produce a large amount of extracellular cellulases, therefore, they have been widely studied and utilized for efficient degradation of lignocellulosic biomass. In this study, we identified two cellulase genes from rotten mandarin peel showing high EG and CBH activities. After determining their cDNA sequences, we expressed these genes in S. cerevisiae under minimal media containing a amorphous cellulose as a sole carbon source and measure cellulolytic activity and ethanol production yield. These results will be contributed to efficient saccharification of various biomasses and further construction of powerful CBP strains in biofuel and biochemical industry. |
