| 초록 |
In tissue engineering, formation of new tissue requires appropriate delivery of tissue-specific cells. A combination of cells with polymer scaffolds has been utilized for cell delivery. Hyaluronate has been frequently used for cartilage regeneration. In this presentation, hyaluronate-alginate hybrid polymers were prepared by using different types of linkers, including ethylenediamine, 1,4-diaminobutane, and 1,8-diaminooctane. As the length of spacer arm between hyaluronate and alginate increased, mechanical stiffness of hybrid hydrogel increased. Hybrid polymer was utilized to fabricate microgel for cartilage regeneration by the emulsion method. Microgel modified with cell-interactive peptides with the sequence of histidine-alanine-valine and arginine-glycine-aspartate formed an aggregate with chondrocytes. This cell/microgel aggregate may be useful for injectable cell delivery in many tissue engineering applications. |
