| 초록 |
Porosity incorporation into calcium phosphate cement (CPC) is a potential attribute to enhance bone regeneration. It effects on cellular migration around the implantation site. Primary focus of our present study was the surface modification of the microchanneled granule by collagen coating and BMP-2 loading and modification of CPC cement by porosity incorporation from the loading of granule. CPC cement was derived from the synergistic effect from monocalcium phosphate monohydrate (MCPM), tetracalcium phosphate (TTCP) and dibasic calcium phosphate anhydrous (DCPA). The optimum powder mix ratio (TTCP: DCPA: MCPM) (molar) was 0.605:0.195:0.2. Fabricated CPC cement is characterized by XRD, scanning electron microscopy (SEM), setting time, injectability, initial pH into media and compressive strength in wet and dry condition. BMP-2 release from granule and set cement was studied. Optimum granule mix was set from compressive strength and surface morphology by SEM. Initial biocompatibility was further investigated from In Vitro study such as MTT, cell proliferation. This set cement was implanted into Rabbit femoral condyle of 8mm critical defect. The efficacy of this complex is evaluated through In Vivo study at 2, 4 and 8 weeks of interval. |
