| 초록 |
The droplet-based microfluidics has led to significant progress in many area including drug development and high-throughput biological assay. The demand for a biological screening assay motivates the development of microfluidic devices as an emerging powerful, efficient alternative to conventional multi-well plate systems, providing a significant benefit by fully separating the distinct biological reactions in very tiny droplets with various combinatorial conditions. In this work, we develop a microfluidic SDA (μSDA) that combines a microvalve system to generate a bacterial population gradient using two different strain into a parallel series of droplets. Under the two bacterial populations, we find the effect of population ratio on cell-cell communications between signal sender (production of signal molecules) and receiver cells (signal molecule receiver). |
