| 초록 |
Inspired by the hierarchically organized protein fibers in extracellular matrix (ECM) as well as the physiological importance of multiscale topography, we developed a simple method for the design and manipulation of precisely controllable multiscale hierarchical structures. In this study, we approached a conceptual platform that can mimic the hierarchically multi-scale topographical and orientation cues of the ECM for controlling cell structure and function. Using our platforms, the structures and orientations of fibroblast cells were greatly influenced by the nanotopography, rather than the micro-topography. We also proposed a new approach that enables the generation of native ECM having nanofibers in specific three-dimensional configurations by culturing fibroblast cells on the multi-scale substrata. We suggest that our methodology could be used as efficient strategies for the design and manipulation of various functional platforms for advanced regenerative medicine applications. |
