| 초록 |
Development of a new substrate for surface-enhanced Raman scattering is one area of interest for the improvement of SERS performance. Herein, we introduce a new method for developing new mesoporous SERS substrates using M13 phages that display cysteine-rich peptides on the pVIII major units. Together with the SERS substrate development, and the use of the SERS technique for sepsis diagnostics is a new approach in clinical settings. The substrates were characterized and magnetized with magnetic immune colloids made of gold-coated magnetic nanoparticles and specific antibodies. The SERS spectra showed distinct peaks for tags corresponding to three typical sepsis-specific biomarkers for diagnostics with the limit of detection values of 27pM, 103pM, and 78pM for CRP, PCT, and sTREM-1, respectively. With such an approach, SERS can be used for clinical purposes and can be improved by phage display modification rather than chemical alternatives. |
