| 초록 |
Surface topography has been one of the most important factor for tissue engineering scaffolds, which can control the cell activities such as cell adhesion, orientation, proliferation, and differentiation. In this study, micro-, yet nano- fibrous scaffolds were produced by self-bundling electrospinning. The surface structure, orientation and its roughness of self-bundled scaffolds were analyzed by SEM and AFM, and other surface properties and mechanical properties were characterized. Moreover, cell behaviors were tested in vitro using PC12 cells, human neural stem cells (hNSCs), and dorsal root ganglia (DRG). Cell adhesion was highly improved in self-bundled scaffold and outstandingly, the physical guidance of the cells and the neurite outgrowth of DRG was promoted. These data indicate the effect of topography of self-bundled scaffold on the cell behaviors and its potential as an advanced electrospun scaffold for tissue regeneration. |
