| 초록 |
Several exceptions of protein sequence-structure relationship is available where protein does not follow the sequence-structure relationship, e.g. GFP-like protein, SH3 like protein, etc. Identification of the conserved residues among the structurally similar protein sequences, with the sequence identity in mid-night zone, has been of keen interest for molecular biologist. In the present study, we attempt to identify the conserved residues that are crucial for protein structure determination at each level of evolution, i.e. sequence evolution, structural evolution and crucial conserved bond. It significantly eliminates the supporting residues which fail to satisfy any of the evolutionary criteria. Number of conserved residues identified by Overlapped conserved residues (OCR) based are relevant to the sequence identity conserved among the diverse members of the protein family. High efficiency of the fold scan analysis, using the sequence pattern obtained from the OCR-based approach, was obtained to detect the similar structural fold. OCR-based approach was successfully tested on GFP like protein were validated using the SPs and Cyanins like protein. |
