| 초록 |
The purpose of this study is to evaluate the osteogenic differentiation of BMSCs on Gallus gallus domesticus derived demineralized bone particle (GDD) scaffolds. GDD scaffolds were prepared by a freeze-drying method using 1, 2 and 3% GDD solution. We characterized GDD scaffolds using SEM, compressive strength, porosity and FT-IR. Also, in vitro evaluation was assessed by MTT assay and ALP activity assay. Also, we confirmed gene expression of OCN, COL1, RUX-2, COX-2 and TNF-α using RT-PCR. Also, we performed in vivo experiment. The tomography was performed using μ-CT. And, histological analysis was performed. Front the results obtained, 3% GDD scaffold was superior and it can be applied as a potential biomaterial for bone regeneration. This research was supported by the International Research & Development Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2017K1A3A7A03089427). |
