| 초록 |
Small interfering RNAs (siRNAs) are double stranded RNAs which have 19-21 nucleic acids. siRNAs incorporate into RNA-induced silencing complex (RISC) and degrade mRNAs which have complementary sequences in a sequence specific manner. In our previous studies, siRNA was conjugated to a PEG via a disulfide linkage (siRNA-PEG) at sense 3’ end. Now we conjugated poly(ethylene glycol) (PEG) to siRNAs at sense 3’end and antisense 5’ end via cleavable and noncleavable linkage. Naked siRNA and siRNA-PEG conjugates were transfected for gene inhibition study using LipofectamineTM2000. Naked siRNA and siRNA-PEG conjugates were transfected into human Peripheral Blood Mononuclear Cell (PBMC) using DOTAP (N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium methylsulfate) to see immune responses. Noncleavable siRNA-PEG conjugates at sense 3’ end did not show any differences in gene inhibition activity and immune responses compared to cleavable ones. |
