| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 가을 (11/08 ~ 11/10, 부산 벡스코(BEXCO)) |
| 권호 |
21권 2호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Antibiotics-free stable and tunable maintenance of multi-copy plasmid by auxotrophic control in Escherichia coli |
| 초록 |
Plasmids require antibiotics for their stable maintenance despite of the economic and environmental problems. Here, we developed the antibiotic-free auxotrophic plasmid maintenance system for stable and tunable gene expression by precise control of plasmid copy number (PCN) in Escherichia coli. To achieve this, we expressed one of essential genes, infA encoding translation initiation factor (IF-1), on plasmid instead of chromosome. With this sytem, the gene expression was stably maintained for 40 generations with minimized cell-to-cell variation under antibiotics-free condition. Moreover, the PCN was rationally tunable in 5-fold by varying expression of infA. Notably, antibiotic-free control of the PCN enabled significant improvement on the production of both itaconic acid (1.60-fold) and lycopene (2.78-fold) compared to that of the conventional system. Collectively, the developed strategy could be a platform for production of value-added products in antibiotic-free cultivation. |
| 저자 |
강채원1, 임현규1, 양진아2, 노명현1, 서상우2, 정규열1
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| 소속 |
1포항공과대, 2서울대 |
| 키워드 |
Plasmid; PCN maintenance; PCN regulation; Essential gene expression level
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| E-Mail |
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