| 초록 |
Glucose homeostasis is tightly controlled in all domains of life including bacteria, such as E. coli. A key regulatory point of glucose homeostasis resides in phosphosugar glucose-6-phosphate (G6P), generated during membrane translocation of glucose by the phosphotransferase system (PTS). Although the charged phosphate group is vital for retention of intracellular glucose, high levels of G6P are toxic to cells. Previous works suggested the mechanism for glucose homeostasis in E. coli with the small regulatory RNA SgrS and the haloacid dehalogenase (HAD)-like enzyme YigL. In this study, we want to decipher glucose homeostasis in four E. coli strains: BL21 (DE3), MC1061, W, and W3110. This work will be expanded our knowledge for glucose homeostasis and understanding physiological implications depending on different genetic backgrounds. |
