| 학회 |
한국화학공학회 |
| 학술대회 |
2018년 봄 (04/25 ~ 04/27, 창원컨벤션센터) |
| 권호 |
24권 1호, p.355 |
| 발표분야 |
생물화공 |
| 제목 |
Poly(2-hydroxyisovalerate-co-lactate) production in Escherichia coli by metabolic engineering method |
| 초록 |
Polyhydroxyalkanoates (PHAs) production in metabolically engineered strains have been studied recently. Here, we show the production of PHAs containing 2-hydroxyisovalerate (2HIV) in Escherichia coli. To generate the 2HIV, feedback resistant ilvBNmut, ilvCD, and panE are amplified. Also, pct540 and phaC1437 are overexpressed for 2HIV synthesis. This strain can produce poly(13.2 mol% 2HIV-co-7.5 mol% 2HB-co-42.5 mol% 3HB-co-36.8 mol% LA), under 20g/L glucose and 2g/L sodium 3-hydroxybutyrate (3HB). To produce sole PHA of 2HIV and LA, poxB, pflB, adhE and frdB genes are deleted. This strain under 20g/L glucose and 2mM L-isoleucine can produce poly(20 mol% 2HIV-co-80 mol% LA) at the polymer content of 9.6% w/w. These results indicate that novel PHAs can be produced with branched-chain amino acid metabolism engineering. [This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science and ICT (MSIT) through the National Research Foundation (NRF) of Korea (NRF-2012M1A2A 2026556 and NRF-2012M1A2A2026557) and NRF grant funded by the MSIT (NRF-2016R1A2B4008707)] |
| 저자 |
구창대1, 양정은1, 김제웅1, 오영훈2, 최소영1, 이 혁2, 오아름2, 신지훈2, 박시재3, 이상엽1
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| 소속 |
1KAIST, 2KRICT, 3이화여자대 |
| 키워드 |
생물화공 |
| E-Mail |
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| 원문파일 |
초록 보기 |
