| 학회 |
한국화학공학회 |
| 학술대회 |
2020년 가을 (10/14 ~ 10/16, e-컨퍼런스) |
| 권호 |
26권 1호, p.974 |
| 발표분야 |
나노기술과 분자진단 심포지엄(생물화공부문위원회) |
| 제목 |
Target-responsive switching of DNA polymerase activity |
| 초록 |
A novel concept that target molecules can induce switching of DNA polymerase activity is devised. The method relies on the finding that a DNA aptamer inhibiting DNA polymerase can undergo conformational change upon interaction with a corresponding target molecule, which leads to the recovery of DNA polymerase. The activated DNA polymerase then promotes the multiple primer extension reactions in a separate TaqMan probe-based signal transduction module, consequently producing significantly enhanced fluorescence signal which can be used to confirm the presence of the target molecules. This design principle was very successfully applied to identify the presence of target DNA, proteins, and even metal ions with high levels of sensitivity and selectivity. We further expanded this innovative concept to develop novel assays for several nucleic acid-related enzymes. |
| 저자 |
박현규 |
| 소속 |
한국과학기술원 |
| 키워드 |
DNA polymerase; Aptamer; Enzyme assay
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| E-Mail |
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| 원문파일 |
초록 보기 |
