| 초록 |
Various types of molecules serve as biomarkers of disease and numerous methods have been reported to quantify them. Recently, research efforts have been made to develop point-of-care tests, which contribute to early diagnoses of diseases, particularly in resource-limited settings. Here, we report a one-step homogeneous assay method based on proximity proteolysis reactions (PPR) for detecting proteins and small molecules. A PPR system has been designed such that the proteolysis between protease and zymogen is enhanced in the presence of a target analyte. The activated zymogen generates a color signal by hydrolyzing a chromophore. The assay methods developed using the PPR have successfully detected several kinds of analytes at subnanomolar concentrations. The one-step procedure and color signal of our PPR-based homogeneous assays will enable the development of simple point-of-care diagnostics for various types of biomarkers. |
