| 초록 |
Recently, exosomes have been actively studied as an alternative to cell based therapy. we designed an experiment to confirm whether exosome of bovine chondrocyte causes chondrogenic differentiation in human mesenchymal stem cells (hMSC) and human adipose derived stem cells (hADSC). we obtained the exosome of chondrocyte through sucrose cushion ultracentrifugation. Next, exosomes were treated with hMSC and hADSC at a dose of 40 ug / mL, and cells with basal media were used as a control. As a result of DNA assay, it was confirmed that exosome was not affect the proliferation of hMSC and hADSC. Collagen type I, collagen type II, and aggrecan, markers of chondrogenic differentiation, were confirmed by RT-PCR. As a result, the exosome groups were not significantly different from the control group in all chondrogenic differentiation markers. Therefore, 40 ug / mL of bovine chondrocyte derived exosome was not cause chondrogenic differentiation in hMSC and hADSC. |
