| 학회 |
한국화학공학회 |
| 학술대회 |
2016년 봄 (04/27 ~ 04/29, 부산 BEXCO) |
| 권호 |
22권 1호, p.456 |
| 발표분야 |
생물화공 |
| 제목 |
In vitro Selection of PDGFR Specific DNA Aptamer for Promotion of Cell Growth |
| 초록 |
Fetal bovine serum (FBS) is the most widely used serum-supplement for the in vitro cell culture, but which is produced expensive and limitation. Furthermore, undefined proteins can lead to unexpected stimulation of cells. The DNA aptamer is oligonucleotide that can bind target molecule. The DNA aptamers, combined with cell growth factor receptor such as platelet-derived growth factor receptor (PDGFR), may promote cell growth. In this study, DNA aptamers generated to specifically bind to PDGFR by using SELEX, real-time PCR and surface plasmon resonance (SPR) spectroscopy. The selected DNA aptamers will measure tyrosine phosphorylation of PDGF receptor by ELISA. This work was supported by the Technological Innovation R&D Program (S2177590) funded by the Small and Medium Business Administration(SMBA, Korea)" |
| 저자 |
김은실1, 차지만1, Simranjeet Singh Sekhon1, 안지영1, 민지호2, 김양훈1
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| 소속 |
1충북대, 2전북대 |
| 키워드 |
대사공학; 동식물세포/조직공학
|
| E-Mail |
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| 원문파일 |
초록 보기 |
