| 초록 |
Present study is focused to provide the effective encapsulation and release of rifampicin, and the synergistic action between rifampicin and amino acid-modified chitosan. In order to examine hydrophobic drug-binding affinity according to hydrophobic structures, hydrophobic amino acids, such as alanine, leucine, isoleucine, tryptophan, phenylalanine, valine, and proline, were grafted to amine groups of chitosan (4.7 kDa)(AGC, LGC, IGC, TGC, FGC, VGC, and PGC, respectively). Rifampicin-loaded chitosan micelles (RCM) are sized in ~50 nm, and showed to spherical forms. Rifampicin was sustainedly released in physiological condition, but was more fast in pathogenic-infection condition, low pH. This effective escape contributed to synergistic antibacterial activity of RCM in vitro and in vivo. This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded the Ministry of Education, Science and Technology (2013R1A1A4A01010701). |
