| 학회 |
한국고분자학회 |
| 학술대회 |
2010년 가을 (10/07 ~ 10/08, 대구 EXCO) |
| 권호 |
35권 2호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
Effect of silk content on the phenotype and proliferation of Retinal Pigment Epithelial cells on silk/PLGA films |
| 초록 |
Biomaterials for retinal tissue engineering must demonstrate several critical features for potential utility in vivo, including mechanical integrity, biocompatibility and slow biodegradation. Silk film biomaterials were designed and characterized to meet these functional requirements. We prepared natural/synthetic hybrid silk/PLGA films using 0, 10, 20, 40 and 80 wt% of silk by the solvent evaporation method. We measured MTT to confirm cellular of cell number attached on film at 1, 2 and 3 days. Morphology of cellular adhesion on films was confirmed by scanning electron microscope (SEM) at 1, 2 and 3 days. RT-PCR was conducted to confirm mRNA expression of RPE65 as RPEs marker and expression of cytokeratin were determined by Immunofluorescence staining. We confirmed that specific 20~40 wt% silk in silk/PLGA film was superior to adhesion and proliferation of RPEs. This research was supported by WCU (R31-20029) . |
| 저자 |
김온유, 조은혜, 김수진, 조수진, 이가영, 이동원, 강길선
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| 소속 |
전북대 |
| 키워드 |
Retinal pigment epithelial cell; silk/PLGA films
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| E-Mail |
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