| 초록 |
Clustered regularly interspaced short palindromic repeat (CRISPR) associated system (Cas9) is one of the most powerful tool that edit target gene with sgRNA. In many studies, therapeutic application of Cas9 is performed by viral vectors due to their high in vivo transfection efficiency. However, many limitations of viral vectors have been reported in connection with clinical trials. Thus, the safe, nonviral delivery of the Cas9 system can lead to significant advances in the use of gene therapy. In this study, we have directly conjugated Cas9 to 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE), which is one of widely used cationic phospholipid substances to improve delivery efficiency with better safety concerns. To determine the genome editing efficiency, we targeted the EGFR oncogene containing a single nucleotide mutation in non-small cell lung cancer. We hope that our approach would provide effective gene editing efficiency and open up new opportunities for clinical trials. |
