| 초록 |
Pyranose oxidase (POx), produced from recombinant E. coli doesn't have non-conductive glycosylation that can interfere with the transfer of electrons, and can also catalyze the oxidation of glucose. In this study, POx was immobilized on carbon nanotubes (CNTs) via three different methods: covalent attachment (CA), enzyme coating (EC) and enzyme precipitate coating (EPC). The activities of POx-CNTs prepared by CA, EC, and EPC methods were 18, 26, and 120 A500/min per 1 mg of CNTs, respectively. The CA, EC and EPC of POx on CNTs were used to fabricate enzymatic electrodes. The sensitivities of the CA, EC, and EPC electrodes without BQ were 0.27, 0.76 and 3.7 mA/M/cm2, respectively, while CA, EC and EPC electrodes with BQ showed 25, 25, and 60 mA/M/cm2 of sensitivities. The immobilization and stabilization of POx via the EPC approach have a great potential that can be used not only for continuous glucose monitoring but also high performance biofuel cells. |
