| 초록 |
We introduce a facile method to fabricate a recyclable cell-alignment scaffold using nanogroove based on liquid crystal (LC) material. Randomly and uniaxially arranged smectic LC structures that have hundreds of nanometers are obtained, followed by the sublimation and recondensation process. We make the nanogroove serve as a scaffold to efficiently induce directed cell growth repeatedly without cytotoxicity by treating osmium tetroxide (OsO4). Together, various cell types, including stem cells, prove the scaffold’s broad applicability. Depending on the nanotopography, cells have different morphologies and gene expression levels compared to cells on standard glass substrates. Furthermore, we developed cell sheets with oriented cells that can be repeatedly formed and transferred to other substrates while maintaining their organization. We believe that our cell-aligning scaffold may give way for the soft material field to bioengineering, involving applications for regenerative medicine. |
