| 학회 | 한국고분자학회 |
| 학술대회 | 2004년 가을 (10/08 ~ 10/09, 경북대학교) |
| 권호 | 29권 2호, p.387 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Development of a mechano-active bioreactor with dynamic compression mode for tissue engineered cartilage |
| 초록 | As articular cartilage is a load-bearing connective tissue, it is subjected to complex loading, including compressive and shearing forces1. It is known that these forces are concerned in the development and maintenance of articular cartilage in the body2. It means the compressive mechanical stimulation is a very important factor for formation of articular cartilage using a tissue engineering technique3. So it is likely to be necessary the development of a mechano-active bioreactor which can give the mechanical signals to adherent cells on polymer scaffolds during mechanical strain application. The objective of this study is to form the engineered cartilaginous constructs in vitro with a mechano-actice bioreactor and to evaluate the effect of dynamic compression for regeneration of cartilage. The scaffolds with 85% porosity and 300~500μm pore size were fabricated with poly(L-lactide-co-ε-carprolactone) copolymer by gel-pressing method. Chondrocytes isolated from rabbit articular cartilage were seeded on scaffolds and cultured for 24 days in the bioreactor. This study shows the application of dynamic compression applied periodically can improve the quality of cartilaginous tissue formed in vitro. Fig. 1. Bioreactor of compressive mode Fig. 2. Quantitative analysis data for examining cartilage tissue formation Reference 1. Stephen D. Waldman, Caroline G. Spiteri, Marc D. Grynpas, Robert M. Pilliar, Jason Hong, Rita A. Kandel, J Bong Joint Surg, 85A(Suppl 2), 101 (2003) 2. Grodzinsky AJ, Levenston ME, Jin M, Frank EH, Annu Rev Biomed Eng, 2, 691 (2000) 3. Eric M. Darling, Kyriacos A. Athanasiou, Annu Biomed Eng, 31, 1114 (2003) |
| 저자 | 정영미1, 유희진1, 김상헌1, 김수현1, 김영하1, Takehisa Matsuda2 |
| 소속 | 1한국과학기술(연), 2규슈대 |
| 키워드 | PLCL; compression; bioreactor; mechanical stimulation |
