| 학회 |
한국공업화학회 |
| 학술대회 |
2016년 봄 (05/02 ~ 05/04, 여수 엑스포 컨벤션) |
| 권호 |
20권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Heterologous expression and functional analysis of methanol and formaldehyde-assimilating enzymes in type I methanotrophs |
| 초록 |
Natural one-carbon metabolic pathways in native hosts such as methylotrophs have recently been reconstructed in non-native hosts mainly in methanol. Engineering strategy for methanol bioconversion was the implementation of NAD-dependent methanol dehydrogenase (MDH) and co-expressing two key enzymes in ribulose monophosphate cycle (RuMP) were hexulose-6-phosphate synthase (Hps) and 6-phospho-3-hexuloisomerase (Phi). MDH and several Hps-Phi gene clusters as well as artificial fusion forms from different type I methanotrophs were cloned and screened. As a results, in vitro activity of mdh and Hps-Phi were detected. The data provided new opportunities for reconstruction of methanol metabolic pathway from methanotrophs into non-native hosts. |
| 저자 |
Nguyen Duc Anh, Nguyen Thi Thu, Nguyen Thi Ngoc Diep, 황인엽, 이은열
|
| 소속 |
Kyung Hee Univ. |
| 키워드 |
NAD-dependent methanol dehydrogenase; methanotroph; RuMP
|
| E-Mail |
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