| 학회 |
한국공업화학회 |
| 학술대회 |
2012년 봄 (05/09 ~ 05/11, 김대중컨벤션센터) |
| 권호 |
16권 1호 |
| 발표분야 |
정밀화학 |
| 제목 |
Dual ligand effect of transferrin and RGD on Avidin-Biotin interaction mediated antitumor peptide delivery |
| 초록 |
In this study, we designed Transferrin antitumor hybrid peptide(Tf-peptide) of Biotinylation (bio-Tf-peptide) and RGD labelled Avidin(RGD-Avidin). We employed an avidin-biotin Dualtargeting approach, in which biotinylated Tf-peptide interaction with RGD-equipped avidin (RGD-avidin-bio-Tf-peptide). RGD-avidin-bio-Tf-peptide were characterized for SDS-PAGE performed on a mini-Protein II System. The mophology of the RGD-avidin-bio-Tf-peptide were observed by Transmission elctron microscopy (TEM). Finally, the cytotoxicity and cellular uptake of RGD-avidin-bio-Tf-peptide against cancer cells were evaluated for their tumor targeting effects using the MTT assay, conforcal laser scanning microscopy(CLSM), and flow cytometry. |
| 저자 |
김태훈, 박준규, 남정표, 김원석, 박성철, 최창용, 장미경, 나재운
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| 소속 |
순천대 |
| 키워드 |
Hybrid antitumor peptide; Transferrin; RGD; Dual targeting; Avidin Biotn interaction
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| E-Mail |
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