| 학회 | 한국재료학회 |
| 학술대회 | 2016년 가을 (11/16 ~ 11/18, 경주 현대호텔) |
| 권호 | 22권 2호 |
| 발표분야 | B. 나노화학/바이오 재료 분과 |
| 제목 | Incorporation of BMP-2 loaded Collagen conjugated multichannel BCP granules in Calcium Phosphate Cement based Injectable Bone Substitutes for improved Bone Regeneration |
| 초록 | ABSTRACT The degradation of calcium phosphate cement (CPC) is essential to complete ossification of implant site for effective bone healing. In this regard, porosity and interconnectivity are key traits of CPC to ease cellular migration and induce favorable osteogenic signaling around the defect site. The objective of the present study was to incorporate surface modified porous multichannel BCP (Biphasic calcium phosphate) granule into CPC system to enhance its in vivo biodegradation and bone tissue growth. CPC was initially fabricated from a mixture of monocalcium phosphate monohydrate (MCPM), tetracalcium phosphate (TTCP), and dibasic calcium phosphate anhydrous (DCPA). Distinct CPCs with varied liquid to powder ratios (0.4, 0.45, and 0.5ml/g) were prepared. The multichannel BCP granule (15wt%) was first coated with collagen subsequent to BMP-2 loading (ccMCG-B). It was then embedded into CPC to form CPC-ccMCG-B system. The newly developed CPC-ccMCG-B system was then examined for analysis of SEM, EDX, XRD, setting time, compressive strength, injectability, pH change, BMP-2 release, in vitro as well as in vivo studies and further compared with CPC. Optimized CPC (0.45ml/g) was found based on setting time and compressive strength studies. The embedment of granules accelerated the CPC degradation and enhanced the bone bone tissue growth with fewer compromise to its compressive strength. In vivo studies exhibited improved bone tissue formation and better degradation of CPC matrix after 2 and 4 weeks of implantation as compared to CPC. New bone formation coupled with faster degradation resulted from effective BMP-2 signaling. Our results suggest that CPC-ccMCG-B system might be used as a promising injectable bone substitutes in clinical applications. |
| 저자 | Gun Hee Lee1, ByongTaek Lee2 |
| 소속 | 1Department of Regenerative Medicine, 2College of Medicine |
| 키워드 | CPC; CPC-ccMCG-B; BCP granule; BMP-2; invivo; invitro |
