| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 봄 (05/12 ~ 05/14, 부산 벡스코(BEXCO)) |
| 권호 |
25권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Development of Automatic Nucleic Acid Testing system for Mycoplasma Monitoring |
| 초록 |
Mycoplsma contamination in the cell culture is one of the most important problems and cumbersome works. Those existance of thoes mycoplamic organisms should be monitred for sucessuful cell culture during culturing. It can be monitored by nulecic acid testing through the real time PCR, which is commercialized already in many bioindustrial companies. However, most of the monitoring kits are based upon manual operation by molecular experts. We have developed a new full-automatice nucleic acid testing machine, in which the total process of the real time PCR could be done by itself. The magnetic bead based DNA extraction process is implemented with a cartridge of all reagents are included and conuously real time PCR process is peformed using the extacted nucleic acid. Turn-around time for a test is about 1 and half hours. This automatice nucleic testing system will reduce the human load for monitoring mycoplasma monitoring during cell culture. |
| 저자 |
박아름1, 박애자1, 이지형1, 김인영1, 이경진1, 정두희1, 김민지1, 김민경1, 박지성1, 김종원1, 한재진2
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| 소속 |
1(주)바이오메듀스, 2(주)셀세이프 |
| 키워드 |
Nucleic acid test; automatic; cell culture; mycoplasma
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| E-Mail |
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