| 초록 |
Single-cells information during cellular responses to external signals is an essential step for quantitative single-cell analyses. The ability to analyze cells at a single-cell level is important when researching cell heterogeneity. The previous methods are limited in their throughput, resolution and accuracy. To address these problem, high resolution live cell images is increasingly used to detect cellular dynamics in response to drugs and chemicals. Microfluidic systems offer advantages of precise fluid control and have recently developed as a method for imaging techniques of single-cells in precisely controlled and changing microenvironments. Here, we have developed a simple microfluidic platform of trapping single-cells in aperture. The device exploits hydrodynamic forces to trap cells flowing near a narrow aperture. We achieved capture of cells with a trapping efficiency of sing-cells over 50 %. The simplicity of the design, inexpensive materials make it a device for systems biology experiment. |
