| 초록 |
Here, we developed interpenetrating (IPN) composite hydrogels containing adipose-derived stem cells (ADSCs) and insulin-like growth factor-1 (IGF-1) loaded coacervates (Coa). It is hypothesized that exogenous IGF-1 delivery via Coa in hydrogels including thiolated gelatin and poly(ethylene glycol)-diacrylate could enhance chondrogenesis of ADSCs and subsequent cartilage repair. Loading efficiency of IGF-1 in Coa was 86 % and a sustained release of IGF-1 was obtained for 21 days. IGF-1 delivery facilitated glycosaminoglycan deposition, indicating enhanced in vitro chondrogenesis of ADSCs. Moreover, hydrogels were implanted into the medial femoral condyle of rabbits and the results showed that a dual delivery group enhanced normal-like cartilage regeneration as compared with any single delivery group. Therefore, IPN composite hydrogels could be utilized as implantable delivery tissue engineered platform for chondrogenesis and cartilage repair. |
