| 학회 |
한국화학공학회 |
| 학술대회 |
2012년 봄 (04/25 ~ 04/27, 제주 ICC) |
| 권호 |
18권 1호, p.398 |
| 발표분야 |
생물화공 |
| 제목 |
Predictive design of mRNA translation initiation region to control prokaryotic translation efficiency |
| 초록 |
Precise prediction of prokaryotic translation efficiency can provide valuable information for optimizing bacterial host for the production of biochemical compounds or recombinant proteins. However, dynamic changes in mRNA folding throughout translation make it difficult to assess translation efficiency. Here, we systematically determined the universal folding regions that affect the efficiency of translation arising via two different initiation mechanisms in Escherichia coli. By assessing the exquisite regions for mRNA folding, we could construct a predictive design method, UTR Designer, and demonstrate that proper codon optimization around the 5’-proximal coding sequence is necessary to achieve a broad range of expression levels. Finally, we applied our method to control the threshold value of input signals switching on a genetic circuit. This should increase our understanding of the processes underlying gene expression and provide an efficient design principle for optimizing various biological systems, thereby facilitating future efforts in metabolic engineering and synthetic biology. |
| 저자 |
서상우, 양재성, 김인해, 양진아, 민병은, 김상욱, 정규열
|
| 소속 |
포항공과대 |
| 키워드 |
5’-untranslated region; mRNA secondary structure; synthetic biology; translation efficiency
|
| E-Mail |
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| VOD |
VOD 보기 |
| 원문파일 |
초록 보기 |
