| 학회 |
한국공업화학회 |
| 학술대회 |
2013년 봄 (05/01 ~ 05/03, ICC JEJU) |
| 권호 |
17권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Expression and characterization of carbonic anhydrase from thermophilic marine bacteria |
| 초록 |
Persephonella marina EX-H1 is chemolithotrophic, thermophilic hydrogen-oxidizing bacterium from a deep sea hydrothermal vent on the East Pacific Ocean and is known to fix CO2 using the A-type reductive tricarboxylic acid (TCA) cycle. Carbonic anhydrase, PMCA, from its genome is homologue of alpha CA from Neisseria gonorrhoeae and shares 50 % identity. Codon optimized sequence has been cloned in pET vectors and its expression has been optimized in E. coli BL21DE3. The purified protein has been characterized and its application in CO2 sequestration has been studied. |
| 저자 |
Bashistha Kamar KANTH, Shipra KUMARI, 민기하, 여기백, 장의경, 유영하, 기미란, 백승필
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| 소속 |
고려대 |
| 키워드 |
Carbonic anhydrase; CO2 sequestration; codon optimization; thermal stability
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| E-Mail |
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