| 초록 |
Combinatorial engineering is one of the most powerful ways to create microbes for economic production of chemicals. The importance of efficient screening has been emphasized to investigate vast phenotypic diversity. However, current methods place constraints on monitoring of intracellular concentration of diverse target products at single cell level. Here, we established a screening platform for assessment of metabolites based on microfluidic static droplet array (SDA) and artificial riboswitch. In this system, a riboswitch visualizes intracellular concentration of a target molecule in a quantitative manner. Then, single cells from a library are individually entrapped in SDA to detect and collect highly productive variants. Using this approach, we analyzed intracellular L-tryptophan concentrations of single Escherichia coli cells. Then, a mutant E. coli library was screened and superior strains were obtained exhibiting up to 145% productivity compared to their parental strain. |
