| 초록 |
Lycopene is a red-colored open-chain unsaturated carotenoid, which is predominantly found in red fruits and vegetables, and widely used as an antioxidant, anti-cancer agent. Combinatorial engineering for direct synthesis of desired products and systems biology to interfere rational metabolic design from complete genome of Escherichia coli have been emerged to enhance lycopene production. In this study gene amplification for balancing precursors i.e. pyruvate and glyceraldehydes-3-phophate in central metabolism are evaluated by bilevel-optimization with enhanced gene modification and lycopene flux using constrained- based FBA and MOMA. As a result of metabolic redesign, metabolic engineered strain produced more lycopene contents. Systematic approach by dual optimization using metabolic flux analysis is indispensable for enhanced lycopene production. (Acknowledgement:This work was financially supported by the Korean Systems Biology Research program (M10309090000-03B5002-00000) of the Korean Ministry of Science and Technology (MOST), and LG chemicals Chair Professorship. Hardware for computational analysis supported by the IBM-SUR program.) |
