| 학회 |
한국화학공학회 |
| 학술대회 |
2015년 봄 (04/22 ~ 04/24, 제주 ICC) |
| 권호 |
21권 1호, p.459 |
| 발표분야 |
생물화공 |
| 제목 |
Bioadsorption of Cu2+ and Ni2+ using Surface Display of Polyhistidine on Bacillus subtilis spore by use of the cotE anchor protein |
| 초록 |
We studied removing heavy metal using Bacillus subtilis spore surface display system. We used cot E protein as an anchoring motif because of its high abundance in coat layer and inserted double 6histidine tag at the C-terminal end of anchoring motif. We checked the surface expression of histidine tag using flow cytometry with FITC labelled anti-his6-antibody. Histogram of flow cytometry showed higher fluorescence intensity, possibly due to their outer location on Bacillus subtilis spore. We tried Cu2+ and Ni2+ adsorption with recombinant spore(CotE-His12) and DB104(wild type) used by atomic adsorption spectrometer. We confirmed that Cu2+ and Ni2+ adsorption capacity of CotE-His12 is higher than DB104(wild type). |
| 저자 |
김준형, 김우일, 박종화
|
| 소속 |
동아대 |
| 키워드 |
Bioadsorption; Surface display
|
| E-Mail |
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| 원문파일 |
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