| 학회 |
한국재료학회 |
| 학술대회 |
2013년 봄 (05/23 ~ 05/24, 여수 엠블호텔(THE MVL)) |
| 권호 |
19권 1호 |
| 발표분야 |
D. 바이오재료(Biomaterials) |
| 제목 |
Incorporation of Freeze-dried Bone Marrow-Derived Mesenchymal Stem Cells in 3-D BCP Scaffold for Tissue Regeneration Applications |
| 초록 |
It has been a trend nowadays to incorporate live stem cells in scaffolds to promote faster tissue regeneration. In this study, freeze-dried mesenchymal stem cells will be loaded in a 3-D BCP scaffold. Being able to preserve longer the stem cells in the scaffold will make it better to facilitate its application in tissue engineering and medical treatment. The BCP scaffold was characterized and its mechanical properties were tested. The cells were cultivated in the scaffold and incubated for 5 days. The scaffold with stem cells were loaded with cryoprotectant then subjected to freeze-drying. After freeze-drying, the scaffolds were stored in -80ºC freezer for 7 days. The presence of MSCs in the pores was evaluated by scanning electron microscope. The cells were then rehydrated and its viability was tested via MTT assay and confocal microscopy. Fresh mesenchymal stem cells were seeded to the scaffold to further test the biocompatibility of the freeze-dried stem cell loaded scaffold. The biocompatibility of the scaffolds was assessed by cell proliferation assays using MTT and gene expression with RT-PCR. Cell adhesion and proliferation were further characterized using SEM and confocal images. |
| 저자 |
Alexander Sadiasa1, Young Ki Min2, Hun Mo Yang3, Byong Taek Lee4
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| 소속 |
1Department of Biomedical Engineering and Materials, 2College of Medicine, 3Soonchunhyang Univ., 4Cheonan-si |
| 키워드 |
Stem cells; Freeze dry; scaffold; bone
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| E-Mail |
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