| 초록 |
Decellularized extracellular matrixes (dECM) have been regarded as an alternative material for tissue/organ regeneration because of their excellent regenerative capacity. Due to their advantages, dECM bioinks have received great attention in the field of 3D bioprinting. Although dECM bioinks exhibit strong tissue regeneration ability, the fabrication of 3D volumetric structures is difficult due to their low shape fidelity and mechanical properties. In this study, we used dECM bioink to create volumetric structures using 3D bioprinting. The granule-based printing reservoir consisting of gelatin slurry was developed for the fabrication of volumetric structures. In this system, rapid pre-gelation of the dECM bioink occurred when the bioink was extruded into the reservoir, facilitating the accumulation of weak bioink into the volumetric constructs with high geometric fidelity. Skeletal muscle constructs were fabricated for verification of this system, and it was confirmed that low hypoxia with minimal cell death occurred in the structures. Highly ordered muscle fibers were produced in 3D bioprinted muscle constructs and showed that they were contracted by electrical stimulation. The muscle constructs exhibited enhanced new muscle formation, innervation, and functional recovery in muscle defect models. This study demonstrates that the granule-based printing reservoir can effectively produce biomimetic tissues using dECM bioinks and suggests the possibility of printing dECM bioinks with low shape fidelity such as liver and brain. |
