| 초록 |
The main goal of this research is to achieve a more efficient production of 1,2-propanediol (1,2-PD) using developed Saccharomyces cerevisiae. 1,2-propanediol (1,2-PD) cannot be produced by wild type Saccharomyces cerevisiae so in order to develop aSaccharomyces cerevisiae mutant which can produce 1,2-PD, mgs gene of E. coli-K12 MG1655 and dhaD gene of Citrobacter freundii were inserted into yeast expression vectors and transformed into the wild type of Saccharomyces cerevisiae. As a result, the batch fermentation of S. cerevisiae YPH500, harboring a mgs gene inserted pJES27 vector, resulted in a yield of 0.17g/L. On the other hand, the methylglyoxal synthase of the recombinant S. cerevisiae YPH500, harboring a dhaD gene inserted pJES29 vector, was inactivated and produced no detectable amount of 1,2-PD. Therefore in order to achieve a maximum yield of 1,2-PD we selected the pESC-TRP vector which is able to co-express several gene. By inserting the dhaD gene into pESC-TRP vector, pJES30 vector was constructed. The maximal yield of 1,2-PD achieved in the 1% galactose batch fermentation by pJES27 and pJES30 harboring S. cerevisiae was 0.45g/L. |
