| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 가을 (10/13 ~ 10/14, 제주 ICC) |
| 권호 | 30권 2호 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Evaluation of polymer scaffolds for tissue engineered intervertebral disc |
| 초록 | Since there is no optimal treatment for the persistent pain associated with intervertebral disc (IVD) degeneration, focus has shifted toward replacement using tissue engineeredIVD. In this study, we are trying an alternative approach that consists of generating a functional IVD composed of disc cells seeded to various scaffolds using tissue-engineering principles. We developed several kinds of scaffolds such as poly(L-lactide-co-glycolide) (PLGA), PLGA/SIS (small intestinal submucosa), PLGA/DBP (demineralized bone particle), PLGA/SIS/DBP, crosslinked SIS sponge, and polyglycolide (PGA) nonwoven mesh. Natural/synthetic hybrid scaffolds as PLGA, SIS/PLGA, DBP/PLGA, and SIS/DBP/PLGA were manufactured by solvent casting/salt leaching method, and crosslinked SIS sponge was fabricated by freeze-drying method. Scaffolds were characterized by SEM and porosimetry. It was evaluated cell proliferation by MTT assay and DNA quantification in vitro and in vivo. Scaffolds seeded rabbit disc cells were implanted into nude mouse and then confirmed by the histologic staining by H&E, Safranin-O, Masson's trichrome and type II collagen immunochemical staining. SIS sponge revealed good DNA production and proliferation of disc cell but the formation of disctissue was incomplete due to fast rate of degradation. Natural biomaterials impregnated PLGA scaffolds have a good potential for the application of tissue engineered disc due to its containing bioactive molecules. Figure1. The type of various scaffolds. Acknowledgment : This work was supported by KMOHW (0405-BO01-0204-0006) and SCRC (SC3100). |
| 저자 | 김순희1, 박기숙1, 최방실1, 하현정1, 이종문1, 양윤선2, 김문석3, 이해방3, 강길선1 |
| 소속 | 1전북대, 2메디포스트, 3한국화학(연) |
| 키워드 | Tissue engineered disc; PLGA; SIS; DBP; hybrid scaffolds |
