| 학회 |
한국고분자학회 |
| 학술대회 |
2011년 봄 (04/07 ~ 04/08, 대전컨벤션센터) |
| 권호 |
36권 1호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
Biodisc Regeneration using Purified Alginate Microcapsulescontaining Human Disc Cells |
| 초록 |
Tissue engineering has the possible to improve upon current techniques for intervertebral disc repair. In this study, purified alginate microcapsules were used in intervertebral disc regeneration. The alginate as a natural polymer that is extracted from seaweeds and purification. Purified alginate microcapsules is fabricated by seed human disc cell into microcapsules. After 1, 3, 7 and 14 day of cell seeding, cell viability activity was measured via MTT assay. Morphological observation, histology, biological assay and PCR were performed. In result, the cell viability were better purified alginate microcapsules than non-purified alginate microcapsules. When we cultured disc cell of human in vitro, we observed better to keep the characteristic of cell in the purified alginate microcapsules. This study suggests that purified alginate microcapsules may serve as a potential cell delivery vehicle. This research was supported by WCU (R31-20029) and XRC (A040004) |
| 저자 |
황지혜1, 김아람1, 김온유1, 윤건호2, 이동원1, 강길선1
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| 소속 |
1전북대, 2가톨릭대 |
| 키워드 |
Alginate; purification; intervertebral disc
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| E-Mail |
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