| 학회 | 한국화학공학회 |
| 학술대회 | 2009년 봄 (04/23 ~ 04/24, 광주 김대중컨벤션센터) |
| 권호 | 15권 1호, p.382 |
| 발표분야 | 생물화공 |
| 제목 | β-carotene production ofrecombinant Escherichia coli with engineered whole mevalonate or non-mevalonate pathway in batch cultures at different temperatures |
| 초록 | B-carotene (C40) functions as a provitamin A, rendering it as functional a food or cosmetics. Recently, recombinant Escherichia coli engineered to contain the whole mevalonate (MVA) pathway as well as foreign genes for -carotene biosynthesis was developed for production of -carotene in our group. In this study, we compared the -carotene producing capacity ofE. coli strains harboring the foreign whole MVN pathway with the ones engineered with non- mevalonate pathway (MEP) at different temperatures ( 370C and 250C) in bioreactor culture controlled at neutral pH. Maximum -carotene production was observed in MVA pathway engineered strain at 370C (119mg/L), while the lowest productivity was found from MEP pathway engineered strain at 250C (46.7 mg/L). In addition, acetate byproduct formation was kept at very low concentration in foreign MVA engineered E. coli at both temperatures compared to the one formed from the non- MVA pathway haborring E. coli. |
| 저자 | 느구엔두쿠앙, 김창준, 김선원, 김성배, 서양곤 |
| 소속 | 경상대 |
| 키워드 | β-carotene; batch fermentation; recombinant Escherichia coli; engineered whole mevalonate pathway; temperature. |
| 원문파일 | 초록 보기 |
