| 학회 |
한국화학공학회 |
| 학술대회 |
2008년 가을 (10/23 ~ 10/24, 부산 BEXCO) |
| 권호 |
14권 2호, p.2701 |
| 발표분야 |
생물화공 |
| 제목 |
Homology model-inspired engineering of marine epoxide hydrolase to enhance the catalytic efficiency for the biosynthesis of chiral epoxides |
| 초록 |
Homology modeling is an efficient method for predicting a structure model from its amino acid sequence based on known protein structures as the template. We applied homology modeling to predict suitable target amino acid residue for molecular engineering of the epoxide hydrolase from marine fish. The epoxide hydrolase from Aspergillus niger was used as the template. The catalytic efficiency of the engineered epoxide hydrolase of marine fish could be enhanced by site-directed mutagenesis of the target residues that identified on the basis of homology modeling with A. niger epoxide hydrolase. Acknowledgement: This work was supported by the Marine and Extreme Genome Research Center Program, Ministry of Land, Transportation and Maritime Affairs, Republic of Korea. |
| 저자 |
이은열1, 최성희2, 김희숙2
|
| 소속 |
1경희대, 2경성대 |
| 키워드 |
homology modeling; epoxide hydrolase; site-directed mutagenesis
|
| E-Mail |
|
| 원문파일 |
초록 보기 |
