| 초록 |
Iron oxide nanoparticles(NPs) were encapsulated with the polyaspartamide based graft copolymers to bind and track the immune cells as imaging probes. The mono-dispersed iron oxide NPs with the mean diameter of 10.7 nm were synthesized by the thermal decomposition method, and their shape and distribution were measured by electrophoretic light scattering and transmission electron microscopy. To enhance their biocompatibility,interfacial and hydrodynamic stability, and fluorescence detection, the biodegradable polysuccinimide (PSI) grafted with several functional groups of octadecylamine(C18),ethanolamine(EA),ethylenediamine (EDA), 4-(N-maleimidomethyl) cyclohexane carboxylic acid N-hydroxysuccinimide ester (SMCC), and fluorescein isothiocyanate (FITC) was coated on the iron oxide NP. The structure of C18/EA/SMCC/FITC-g-PSI copolymer was confirmed using 1H-NMR and FTIR spectroscopy, and its cell binding ability was investigated by the flow cytometry and confocal laser scanning microscope. |
