초록 |
Microalgae are fascinating bio-platforms capable of simultaneously mitigating CO2 emissions and producing various high-value products. Nevertheless, their productivity remains insufficient for commercialization due to the irregularity of sunlight in field culture conditions. To overcome these obstacles, we rapidly selected fast-growing mutant strains under fluctuating light from a mutant library via high-throughput sorting of high-density microdroplets. The selected mutant was applied to large-scale outdoor cultivation utilizing sunlight and flue gas CO2. Consequently, without the supplementation of additional growth factors, the mutant strain exhibited increased biomass productivity by 34.38% and increased lipid content by 33.69% relative to the wild-type strain. It is noteworthy that lipid-rich microalgae biomass can be utilized as combustion fuel without additional post-treatment processes, which can be an effective strategy considering carbon re-emission in downstream processes. Our proposed multilateral approach can achieve maximum productivity in outdoor culture, which has been a challenging obstacle to microalgae-based CO2 bioconversion. |